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KMID : 1007520100190020565
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Food Science and Biotechnology
2010 Volume.19 No. 2 p.565 ~ p.569
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Gene Cloning and Characterization of a Trehalose Synthase from Corynebacterium glutamicum ATCC13032
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Kim Tae-Kyun
Jang Jun-Hyuck
Cho Hong-Yeon
Lee Heung-Shick
Kim Young-Wan
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Abstract
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Trehalose synthase (TreS) is an enzyme which produces trehalose from maltose through intramolecular transglycosylation. In this study, a gene (cg2529) encoding for TreS from Corynebacterium glutamicum (CgTS) was cloned and expressed in Escherichia coli. The hexahistidinetagged CgTS showed an optimum temperature and pH of 35oC and pH 7.0, respectively. This enzyme was not thermostable, but stable in a broad pH range from pH 5.0 to 8.5. Its activity slightly increased by 5 mM Mg2+ and Fe2+, while it was strongly inhibited by 5 mM sodium dodecyl sulfate (SDS). CgTS catalyzed the conversion from maltose into trehalose, and vice versa. Lowering reaction temperature by 5oC from the optimum temperature significantly reduced hydrolysis activity to produce glucose as a by-product compared to transglycosylation activity to produce trehalose, leading to increase in the conversion yields from maltose into trehalose. Consequently, the maximum conversion yield by CgTS reached 69% at 25oC after 9 hr of reaction.
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KEYWORD
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trehalose, Corynebacterium glutamicum, trehalose synthase, intramolecular transglycosylation
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